Technical Description
The GenAmplify™ DNA Purification Kit, including Blood, Clinic, and Cell SV kit provide fast and easy methods for the small-scale purification of total DNA from various sample matrices such as blood, tissues, bacterial or cultured cells, and forensic specimens. Purified DNA can be used directly for PCR, Southern blotting, and other downstream applications.
The GenAmplify™ DNA Purification Kit utilizes the advanced silica-binding technology to purify total DNA sufficiently pure for many applications. Various samples are lysed in the optimized buffer containing detergents and lytic enzyme. Under high salt condition, DNA in the lysate bind to the silica membrane and impurities pass through the membrane into a collection tube. The membranes are washed with a series of alcohol-containing buffers to remove any traces of proteins, cellular debris, and salts. Finally, pure DNA is released into a clean collection tube with deionized water or low ionic strength buffer.